OrthoInspector is a package dedicated to orthology inference and analysis. The package can be installed for local use on a desktop computer or configured for a server implementation. Its purpose is to facilitate the installation of a database of newly inferred orthology relations and facilitate the exploitation of the resulting orthology database by both expert and non-expert users.
OrthoInspector is compatible with all systems supporting Java 1.6.x JVM and superior. Its components are based on a SQL database / Java client architecture. Consequently, you need to setup an access to an SQL engine (locally or remotely). Any database engine can be used as soon as a Java JDBC driver is available for it (see complete Requirements for details). Exploiting the inferred orthologs will also require a local installation of the latest NCBI blast+ package.
While the package can be installed on a single machine, we choose to code OrthoInspector in Java and through a SQL database / Java client architecture to facilitate its exploitation in a network environment. Orthology relations are first predicted from a set of proteomes and a blast all-against-all. They are transferred to an SQL database and can be exploited by several clients, allowing several users to query the database via a graphical interface or allowing several pipelines to exploit these data simultaneously.
Be aware that OrthoInspector can be installed either on a network or on a single desktop computer ! You just need to setup an accessible SQL engine, locally (desktop computer) or remotely (office network). The network configuration described below is a recommendation but can be adapted to your needs.
The package is separated in three Java executables (and a few configuration files) :
Installation command-line: A command-line to predict orthology relations and transfer them in a SQL database.
Query command-line: A command-line to query these predictions or export them from the database.
Graphical interface: a desktop interface which regroup most high-level functionnalities (complex queries and visualisation tools) but can also be used directly to infer small orthology datasets (<100 species).
Both the Installation command-line and the Graphical interface can be used to infer orthology relations. The query command-line and most other functions from the graphical interface are designed to exploit these predictions.
This component is recommended to infer large orthology databases from large datasets (> 100 species). A similar procedure is available in the graphical interface but the Installation command line provides several useful options to handle larger datasets. For instance, it can read compressed blast inputs, create compressed outputs, share predictions to several CPUs and facilitate crash recovery (useful when your calculations require a weeks of CPU...).
This component is designed for basic data retrieval from the orthology database. You can query the databases with textual searches, fasta queries or export the entire set of orthologs related to a particular organism. It can easily be integrated into other bioinformatic pipelines.
Contains most functionalities and is a good option for non-specialists which want to avoid command-line manipulations (but you still need to install and configure the SQL database). You can install a small database (<100 organisms) with an automated procedure. This procedure is fully automated if you use MySQL or Postgresql engines. The graphical interface contains advanced querying tools, such as sequence extractions based on phylogenetic profiles. All the graphical tools dedicated to orthology analysis and visualization (Euler diagrams, automated phylogenetic profile construction...) are also in the Graphical interface.
To know more about these tools, consult the dedicated tutorials which are available on this website.
We have pre-computed 4 orthology databases, exploring most known clades (see the Databases tab for more information):
OrthoInspector Cross-domains: a general database covering the three domains of life. 144 eukaryotes, 142 bacteria and 31 archaea.
OrthoInspector Eukaryota: specific eukaryotic database with 711 species.
OrthoInspector Bactera: specific bacteria database with 3,863 species.
OrthoInspector Archaea: specific archaea database with 179 species.
All databases can be accessed and browsed from the "database" menu of this website. The online databases doesn't provide the extended functions of the OrthoInspector package but allow you to retrieve orthology relationships of a given protein in these databases. The web interface offers basic analysis tool and the possibility to easily export results and perform multiple sequences alignement via PipeAlign2 (Kress et al, in prep).
OrthoInspector is licensed under the GNU GPL version 3. You can download it, modify it and share it at your convenience but don't forget to cite the authors ! ;-)